Diagnosis by PCR
NewsPolymerase chain reaction (PCR) is a method that allows you to increase the small number of DNA fragments in a sample. This method was invented by biochemist Cary Mullis in 1983, thanks to which he received the Nobel Prize in Chemistry. This discovery allowed us to solve many problems.
How is PCR used?
- diagnosis of hereditary diseases;
- identification of genetic features associated with individual susceptibility to drugs;
- diagnosis of viral diseases;
- establishing paternity;
- identification of the criminal;
- as one of the stages of more complex diagnostic methods, etc.
What is PCR?
After the DNA extraction stage, the laboratory specialist mixes the components of the reaction mixture, which includes:
● Sample to be analyzed;
● Specially selected nucleotide sequences (primers);
● Thermally stable DNA polymerase – a special enzyme that allows the reaction to take place;
● Multi-component buffer solution.
This reaction is carried out using special devices - PCR amplifiers. And it is divided into the following stages:
1. the process of transition of the double helix of DNA into single chains (denaturation);
2. attachment of primers to single DNA chains (annealing);
3. completion of the second DNA chain (elongation, or synthesis).
This cycle of stages is repeated. After the end of the reaction, the specialist analyzes the results obtained.
Currently, the PCR method is widely used in medical practice due to a number of advantages:
· Relative speed of getting the result (from 3 hours)
· High specificity
· Versatility
· High sensitivity
* Reliability of the results obtained
If you still have questions, we will be happy to answer you by phone: +7 905 525 50 05 or email: person@mknc.ru
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